US $300, UK £175 * – single user license – online fulfillment
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Turns an Ordinary Office Scanner into a Powerful Densitometer for the Analysis of
Gels, Blots, TLC Plates, etc.
QuantiScan for Windows has similar functionality to sophisticated
densitometers but at a fraction of the cost. It is able to analyze
a wide variety of material including polyacrylamide and agarose gels,
autoradiograms, TLC plates, etc. The program will accept images for
analysis directly from any TWAIN compliant scanner as well as most
BMP, JPG or uncompressed TIF files. For laned gels of reasonable
quality, the analysis is highly automated, allowing extremely high
throughput of data. Almost any type of image can be analyzed with
the manual modes of Quantiscan. Images can be pre-processed in a
variety of ways within QuantiScan including crop, flip, invert,
scroll and zoom.
Numeric, graphical and image output can be printed as reports and can
also be saved as files or copied to the clipboard. The X,Y coordinates
of densitometric profiles can output into a tabbed table for re-plotting
in presentation graphics software such as Biosoft's FigSys. There is a
separate module for analysis of discrete blots which can also be operated
with automatic or manual blot detection.
Lane Analysis Mode
There is sophisticated lane processing and analysis for gels.
Lanes of any width can be created manually or by using the unique
automatic lane detection mode. There is also a facility to automatically
set lanes at regular intervals for use with plates that have a
preconfigured regular lane array. Lanes can be skewed or curved
to allow for gel imperfections. Lane boundaries can be copied and
the copies moved precisely into position with the cursor keys, for
exact comparisons. Each lane can be labelled with an unique number
both on its image and its densitometric profile plot. There are six
options for automatic background subtraction: Minimum, Interpolated
minimum, Valley, Edge, Polynomial and Manual. User-selected areas
of images can be displayed as 3-D density maps which can be printed,
saved or copied to the clipboard.
Once a lane has been defined, its densitometric profile plot is
automatically drawn. Peaks can then be specified on the profile
either manually by marking their center and edges, or automatically
using a sophisticated peak search routine. Where appropriate,
automatic fitting of Gaussian peaks can be performed. This
automatic fitting uses a least-squares minimization technique
to fit a sequence of peaks and a polynomial background to the given
profile. The image of the currently-selected lane is shown laid-out
horizontally under the lane densitometry profile. A cursor-controlled
vertical line going across the profile and the lane image aids user
examination of the peaks. The image and profile of a standard lane
can be added for comparison with the first. Their alignment can be
adjusted to aid accurate side-by-side comparisons. Peak areas are
calculated by integration using Simpson's rule and, if the automatic
fitting option has been used, the area of the fitted peak is also
Calibration to a lane containing standard proteins may be made for
automatic calculation of molecular weights. Calibration can also be
made with a known standard solution such as those supplied with
assay kits (just enter the position of its start line, its mol.
wt. factor and the position of the solvent front, to give automatic
calculation of mol. wts of unknowns). Parameters of any standard lane
can be saved for between-plate comparisons. Calibration curve graphs
and their fitted coordinates can be exported. Reports are generated
for all peak parameters, including position, height, width, background
and net area of peaks and, together with the graphical results, these
can be printed, saved to disk or copied to the clipboard for transfer
to other programs.
Blot Analysis Mode
QuantiScan offers a separate mode to analyze discrete blots.
The blots can be automatically detected or manually defined.
Their number, position, height, area and densitometric volume
are automatically reported. There is a special module for automatic
analysis of regularly arrayed blots. Automatic background subtraction
is an option. Calibration, based on densitometric volume of standard
blots can be done and this allows blot mode to be used for assays.
* Single user license US $300/ UK £175
5 user license US $900/ UK £525
10 user license US $1500/UK £875
The above prices are for online fulfillment only and include user manual as .pdf
An additional charge of $90/£50 is made for supply of hard copy (CD) if required